Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome.

ABSTRACT:

Background

Brucellosis is a major bacterial zoonosis affecting domestic livestock and wild mammals, as well as humans around the globe. While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.

Results

The proteomic data was compiled from several independent comparative studies of Brucella abortus that used either outer membrane blebs, cytosols, or whole bacteria grown in media, as well as intracellular bacteria recovered at different times following macrophage infection. We identified a total of 621 bacterial proteins that were differentially expressed in a condition-specific manner. For 305 of these proteins we provide the first experimental evidenc

SUBMITTER: Lamontagne J 

PROVIDER: S-EPMC2877026 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature