Snail transcription factor regulates neuroendocrine differentiation in LNCaP prostate cancer cells.

ABSTRACT:

Background

Snail transcription factor induces epithelial-mesenchymal transition (EMT) via decreased cell adhesion-associated molecules like E-cadherin, and increased mesenchymal markers like vimentin. We previously established Snail-mediated EMT model utilizing androgen-dependent LNCaP cells. These cells express increased vimentin protein and relocalization of E-cadherin from the cell membrane to the cytosol. Interestingly, Snail transfection in LNCaP cells resulted in cells acquiring a neuroendocrine (NE)-like morphology with long neurite-like processes.

Methods

We tested for expression of NE markers neuron-specific enolase (NSE) and chromogranin A (CgA) by Western blot analysis, and performed proliferation assays to test for paracrine cell proliferation.

Results

LNC

SUBMITTER: McKeithen D 

PROVIDER: S-EPMC2877267 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature