Ontology highlight
ABSTRACT:
SUBMITTER: Boughattas S
PROVIDER: S-EPMC2877409 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Boughattas Sonia S Ben-Abdallah Rym R Siala Emna E Souissi Olfa O Aoun Karim K Bouratbine Aïda A
The American journal of tropical medicine and hygiene 20100601 6
Here, we determined the Toxoplasma gondii genotype in amniotic fluid, placenta, and cerebrospinal fluid samples from 14 congenital toxoplasmosis cases in Tunisia, North Africa. Direct genotypic characterization of T. gondii strains was performed by polymerase chain reaction (PCR) amplification of six genetic markers (3'SAG2, 5' SAG2, SAG3, BTUB, GRA6, and APICO) and thereafter, was analyzed by restriction fragment-length polymorphism (RFLP). Samples were sequenced to resolve strain type whenever ...[more]