Evolutionarily conserved replication timing profiles predict long-range chromatin interactions and distinguish closely related cell types.

ABSTRACT: To identify evolutionarily conserved features of replication timing and their relationship to epigenetic properties, we profiled replication timing genome-wide in four human embryonic stem cell (hESC) lines, hESC-derived neural precursor cells (NPCs), lymphoblastoid cells, and two human induced pluripotent stem cell lines (hiPSCs), and compared them with related mouse cell types. Results confirm the conservation of coordinately replicated megabase-sized "replication domains" punctuated by origin-suppressed regions. Differentiation-induced replication timing changes in both species occur in 400- to 800-kb units and are similarly coordinated with transcription changes. A surprising degree of cell-type-specific conservation in replication timing was observed across regions of conserved synten

SUBMITTER: Ryba T 

PROVIDER: S-EPMC2877573 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature