A revisit to the one form kinetic model of prothrombinase.

ABSTRACT: Thrombin is generated enzymatically from prothrombin by two pathways with the intermediates of meizothrombin and prethrombin-2. Experimental concentration profiles from two independent groups for these two pathways have been re-analyzed. By rationally combining the independent data sets, a simple mechanism can be established and rate constants determined. A structural model is consistent with the data-derived finding that mechanisms that feature channeling or ratcheting are not necessary to describe thrombin production.

SUBMITTER: Lee CJ 

PROVIDER: S-EPMC2877601 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature