Quantitative polymerase chain reaction analysis by deconvolution of internal standard.

ABSTRACT:

Background

Quantitative Polymerase Chain Reaction (qPCR) is a collection of methods for estimating the number of copies of a specific DNA template in a sample, but one that is not universally accepted because it can lead to highly inaccurate (albeit precise) results. The fundamental problem is that qPCR methods use mathematical models that explicitly or implicitly apply an estimate of amplification efficiency, the error of which is compounded in the analysis to unacceptable levels.

Results

We present a new method of qPCR analysis that is efficiency-independent and yields accurate and precise results in controlled experiments. The method depends on a computer-assisted deconvolution that finds the point of concordant amplification behavior between the "unknown" template and an

SUBMITTER: Hirakawa Y 

PROVIDER: S-EPMC2877679 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature