BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences.

ABSTRACT:

Background

Bisulfite sequencing is a popular method to analyze DNA methylation patterns at high resolution. A region of interest is targeted by PCR and about 20-50 subcloned DNA molecules are usually analyzed, to determine the methylation status at single CpG sites and molecule resolution.

Results

The BISMA (Bisulfite Sequencing DNA Methylation Analysis) software for analysis of primary bisulfite sequencing data implements sequencing data extraction and enhanced data processing, quality controls, analysis and presentation of the methylation state. It uses an improved strategy for detection of clonal molecules and accurate CpG site detection and it supports for the first time analysis of repetitive sequences.

Conclusions

BISMA works highly automated but still provides

SUBMITTER: Rohde C 

PROVIDER: S-EPMC2877691 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature