Epistasis of transcriptomes reveals synergism between transcriptional activators Hnf1alpha and Hnf4alpha.

ABSTRACT: The transcription of individual genes is determined by combinatorial interactions between DNA-binding transcription factors. The current challenge is to understand how such combinatorial interactions regulate broad genetic programs that underlie cellular functions and disease. The transcription factors Hnf1alpha and Hnf4alpha control pancreatic islet beta-cell function and growth, and mutations in their genes cause closely related forms of diabetes. We have now exploited genetic epistasis to examine how Hnf1alpha and Hnf4alpha functionally interact in pancreatic islets. Expression profiling in islets from either Hnf1a(+/-) or pancreas-specific Hnf4a mutant mice showed that the two transcription factors regulate a strikingly similar set of genes. We integrated expression and genomic binding

SUBMITTER: Boj SF 

PROVIDER: S-EPMC2877749 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature