Ontology highlight
ABSTRACT:
SUBMITTER: Taylor JEN
PROVIDER: S-EPMC2877798 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Taylor James E N JEN Callow Phil P Swiderska Anna A Kneale G Geoff GG
Journal of molecular biology 20100317 3
The Type I R-M system EcoR124I is encoded by three genes. HsdM is responsible for modification (DNA methylation), HsdS for DNA sequence specificity and HsdR for restriction endonuclease activity. The trimeric methyltransferase (M<sub>2</sub>S) recognises the asymmetric sequence (GAAN<sub>6</sub>RTCG). An engineered R-M system, denoted EcoR124I<sub>NT</sub>, has two copies of the N-terminal domain of the HsdS subunit of EcoR124I, instead of a single S subunit with two domains, and recognises the ...[more]