Two-hybrid analysis of Ty3 capsid subdomain interactions.

ABSTRACT:

Background

The yeast retrotransposon Ty3 forms stable virus-like particles. Gag3, the major structural protein, is composed of capsid, spacer and nucleocapsid domains. The capsid domain of Gag3 was previously modeled as a structure similar to retrovirus capsid.

Findings

Two-hybrid analysis was used to understand the interactions that contribute to particle assembly. Gag3 interacted with itself as predicted based on its role as the major structural protein. The N-terminal subdomain (NTD) of the capsid was able to interact with itself and with the C-terminal subdomain (CTD) of the capsid, but interacted less well with intact Gag3. Mutations previously shown to block particle assembly disrupted Gag3 interactions more than subdomain interactions.

Conclusions

The findings

SUBMITTER: Zhang M 

PROVIDER: S-EPMC2878294 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature