Targeted gene deletions in C. elegans using transposon excision.

ABSTRACT: We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

SUBMITTER: Frokjaer-Jensen C 

PROVIDER: S-EPMC2878396 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature