Mammalian numb-interacting protein 1/dual oxidase maturation factor 1 directs neuronal fate in stem cells.

ABSTRACT: In this study, we describe a role for the mammalian Numb-interacting protein 1 (Nip1) in regulation of neuronal differentiation in stem cells. The expression of Nip1 was detected in the developing mouse brain, embryonic stem cells, primary neuronal stem cells, and retinoic acid-treated P19 embryonal carcinoma cells. The highest expression of Nip1 was observed in undifferentiated neuronal stem cells and was associated with Duox1-mediated reactive oxygen species ROS production. Ectopic nip1 expression in P19 embryonal carcinoma cells induced neuronal differentiation, and this phenotype was also linked to elevated ROS production. The neuronal differentiation in nip1-overexpressing P19 cells was achieved in a retinoic acid-independent manner and was corroborated by an increase in the expressio

SUBMITTER: Kennedy KA 

PROVIDER: S-EPMC2878559 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature