Structure-guided mutational analysis of a yeast DEAD-box protein involved in mitochondrial RNA splicing.

ABSTRACT: DEAD-box proteins are RNA-dependent ATPase enzymes that have been implicated in nearly all aspects of RNA metabolism. Since many of these enzymes have been shown to possess common biochemical properties in vitro, including the ability to bind and hydrolyze ATP, to bind nucleic acid, and to promote helix unwinding, DEAD-box proteins are generally thought to modulate RNA structure in vivo. However, the extent to which these enzymatic properties are important for the in vivo functions of DEAD-box proteins remains unclear. To evaluate how these properties influence DEAD-box protein native function, we probed the importance of several highly conserved residues in the yeast DEAD-box protein Mss116p, which is required for the splicing of all mitochondrial catalytic introns in Saccharomyces cerevi

SUBMITTER: Bifano AL 

PROVIDER: S-EPMC2878758 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature