Apparent activity in high-throughput screening: origins of compound-dependent assay interference.

ABSTRACT: Expansive compound collections made up of structurally heterogeneous chemicals, the activities of which are largely undefined, present challenging problems for high-throughput screening (HTS). Foremost is differentiating whether the activity for a given compound in an assay is directed against the targeted biology, or is the result of surreptitious compound activity involving the assay detection system. Such compound interference can be especially difficult to identify if it is reproducible and concentration-dependent - characteristics generally attributed to compounds with genuine activity. While reactive chemical groups on compounds were once thought to be the primary source of compound interference in assays used in HTS, recent work suggests that other factors, such as compound aggregat

SUBMITTER: Thorne N 

PROVIDER: S-EPMC2878863 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature