Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones.

ABSTRACT: Nucleosome disruption and replacement are crucial activities that maintain epigenomes, but these highly dynamic processes have been difficult to study. Here, we describe a direct method for measuring nucleosome turnover dynamics genome-wide. We found that nucleosome turnover is most rapid over active gene bodies, epigenetic regulatory elements, and replication origins in Drosophila cells. Nucleosomes turn over faster at sites for trithorax-group than polycomb-group protein binding, suggesting that nucleosome turnover differences underlie their opposing activities and challenging models for epigenetic inheritance that rely on stability of histone marks. Our results establish a general strategy for studying nucleosome dynamics and uncover nucleosome turnover differences across the genome tha

SUBMITTER: Deal RB 

PROVIDER: S-EPMC2879085 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature