Direct bacterial killing in vitro by recombinant Nod2 is compromised by Crohn's disease-associated mutations.

ABSTRACT:

Background

A homeostatic relationship with the intestinal microflora is increasingly appreciated as essential for human health and wellbeing. Mutations in the leucine-rich repeat (LRR) domain of Nod2, a bacterial recognition protein, are associated with development of the inflammatory bowel disorder, Crohn's disease. We investigated the molecular mechanisms underlying disruption of intestinal symbiosis in patients carrying Nod2 mutations.

Methodology/principal findings

In this study, using purified recombinant LRR domains, we demonstrate that Nod2 is a direct antimicrobial agent and this activity is generally deficient in proteins carrying Crohn's-associated mutations. Wild-type, but not Crohn's-associated, Nod2 LRR domains directly interacted with bacteria in vitro, altered

SUBMITTER: Perez LH 

PROVIDER: S-EPMC2879363 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature