In vitro and in vivo inhibition of neuroblastoma tumor cell growth by AKT inhibitor perifosine.

ABSTRACT:

Background

Activated AKT is a marker of decreased event-free or overall survival in neuroblastoma (NB) patients. The aim of this study was to investigate the effect of perifosine, a nontoxic AKT inhibitor, as a single agent on NB cell growth in vitro and in vivo.

Methods

Four human NB cell lines (AS, NGP, BE2, and KCNR) were treated with increasing concentrations of perifosine, and a quantitative analysis of cell death (apoptosis) was performed by using MTS and caspase-3/7 activity assays. Survival of mice carrying xenograft NB tumors that were treated with perifosine (n = 6-7 mice per group) was compared with that of untreated mice (n = 7 mice per group) using Kaplan-Meier analysis. Tumor volumes were calculated to determine the effect of perifosine on NB tumor growth. Phos

SUBMITTER: Li Z 

PROVIDER: S-EPMC2879416 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature