Teolenn: an efficient and customizable workflow to design high-quality probes for microarray experiments.

ABSTRACT: Despite the development of new high-throughput sequencing techniques, microarrays are still attractive tools to study small genome organisms, thanks to sample multiplexing and high-feature densities. However, the oligonucleotide design remains a delicate step for most users. A vast array of software is available to deal with this problem, but each program is developed with its own strategy, which makes the choice of the best solution difficult. Here we describe Teolenn, a universal probe design workflow developed with a flexible and customizable module organization allowing fixed or variable length oligonucleotide generation. In addition, our software is able to supply quality scores for each of the designed probes. In order to assess the relevance of these scores, we performed a real hybr

SUBMITTER: Jourdren L 

PROVIDER: S-EPMC2879536 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature