Absence of CD34 on murine skeletal muscle satellite cells marks a reversible state of activation during acute injury.

ABSTRACT:

Background

Skeletal muscle satellite cells are myogenic progenitors that reside on myofiber surface beneath the basal lamina. In recent years satellite cells have been identified and isolated based on their expression of CD34, a sialomucin surface receptor traditionally used as a marker of hematopoietic stem cells. Interestingly, a minority of satellite cells lacking CD34 has been described.

Methodology/principal findings

In order to elucidate the relationship between CD34+ and CD34- satellite cells we utilized fluorescence-activated cell sorting (FACS) to isolate each population for molecular analysis, culture and transplantation studies. Here we show that unless used in combination with alpha7 integrin, CD34 alone is inadequate for purifying satellite cells. Furthermore, t

SUBMITTER: Ieronimakis N 

PROVIDER: S-EPMC2880004 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature