Positive selection of DNA-protein interactions in mammalian cells through phenotypic coupling with retrovirus production.

ABSTRACT: Through the shuffling of predefined modular zinc finger domains with predictable target site recognition in vitro, we have generated a large repertoire of artificial transcription factors with five zinc finger domains (TF(ZF)s). Here we report an effective strategy for the selection of ATF libraries by coupling expression of transcriptional activators of the promoter of interest to the enhanced production of retroviral vector particles transferring the TF(ZF) encoding gene. Using this strategy, we successfully selected specific TF(ZF)s that upregulate the expression of the gamma-globin promoter. Selected transcription factors induced the expression of gamma-globin when coupled to an activation domain and reduced expression when linked to a repression domain. This new retroviral approach mi

SUBMITTER: Tschulena U 

PROVIDER: S-EPMC2880176 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature