In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells.

ABSTRACT:

Objective

CD34(+) cells, present within the bone marrow, have previously been shown to possess pancreatic endocrine potential. Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine capacity.

Materials and methods

Sheep were transplanted with hESC-derived CD34(+) cells, as well as nonsorted differentiated cultures. Transplantations were carried out with in utero intraperitoneal injections prior to development of the immune system in the fetus so that tolerance toward foreign antigens was acquired during gestation and persisted in the adult.

Results

All cell populations that were tested demonstrated human cellular activity and long-term

SUBMITTER: Goodrich AD 

PROVIDER: S-EPMC2880573 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature