PRODUCTION OF NITRIC OXIDE WITHIN THE APLYSIA CALIFORNICA NERVOUS SYSTEM.

ABSTRACT: Nitric oxide (NO), an intercellular signaling molecule, helps coordinate neuronal network activity. Here we examine NO generation in the Aplysia central nervous system using 4,5-diaminofluorescein diacetate (DAF-2 DA), a fluorescent reagent that forms 4,5-diaminofluorescein triazole (DAF-2T) upon reaction with NO. Recognizing that other fluorescence products are formed within the biochemically complex intracellular environment, we validate the observed fluorescence as being from DAF-2T; using both capillary electrophoresis and mass spectrometry we confirm that DAF-2T is formed from tissues and cells exposed to DAF-2 DA. We observe three distinct subcellular distributions of fluorescence in neurons exposed to DAF-2 DA. The first shows uniform fluorescence inside the cell, with these cells b

SUBMITTER: Ye X 

PROVIDER: S-EPMC2880821 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature