Comparative proteomic analysis of plasma membrane proteins between human osteosarcoma and normal osteoblastic cell lines.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common primary malignant tumor of bone in children and adolescents. However, the knowledge in diagnostic modalities has progressed less. To identify new biomarkers for the early diagnosis of OS as well as for potential novel therapeutic candidates, we performed a sub-cellular comparative proteomic research.

Methods

An osteosarcoma cell line (MG-63) and human osteoblastic cells (hFOB1.19) were used as our comparative model. Plasma membrane (PM) was obtained by aqueous two-phase partition. Proteins were analyzed through iTRAQ-based quantitative differential LC/MS/MS. The location and function of differential proteins were analyzed through GO database. Protein-protein interaction was examined through String software. One of differentiall

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC2880991 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature