A protein phosphorylation threshold for functional stacking of plant photosynthetic membranes.

ABSTRACT: Phosphorylation of photosystem II (PSII) proteins affects macroscopic structure of thylakoid photosynthetic membranes in chloroplasts of the model plant Arabidopsis. In this study, light-scattering spectroscopy revealed that stacking of thylakoids isolated from wild type Arabidopsis and the mutant lacking STN7 protein kinase was highly influenced by cation (Mg(++)) concentrations. The stacking of thylakoids from the stn8 and stn7stn8 mutants, deficient in STN8 kinase and consequently in light-dependent phosphorylation of PSII, was increased even in the absence of Mg(++). Additional PSII protein phosphorylation in wild type plants exposed to high light enhanced Mg(++)-dependence of thylakoid stacking. Protein phosphorylation in the plant leaves was analyzed during day, night and prolonged d

SUBMITTER: Fristedt R 

PROVIDER: S-EPMC2881038 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature