Open MS/MS spectral library search to identify unanticipated post-translational modifications and increase spectral identification rate.

ABSTRACT:

Motivation

Identification of post-translationally modified proteins has become one of the central issues of current proteomics. Spectral library search is a new and promising computational approach to mass spectrometry-based protein identification. However, its potential in identification of unanticipated post-translational modifications has rarely been explored. The existing spectral library search tools are designed to match the query spectrum to the reference library spectra with the same peptide mass. Thus, spectra of peptides with unanticipated modifications cannot be identified.

Results

In this article, we present an open spectral library search tool, named pMatch. It extends the existing library search algorithms in at least three aspects to support the identification

SUBMITTER: Ye D 

PROVIDER: S-EPMC2881370 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature