Inorganic arsenic as a developmental toxicant: in utero exposure and alterations in the developing rat lungs.

ABSTRACT: In the present study, we characterize the toxic effects of in utero arsenic exposure on the developing lung. We hypothesize that in utero exposure to inorganic arsenic through maternal drinking water causes altered gene and protein expression in the developing lung, indicative of downstream molecular and functional changes. From conception to embryonic day 18, we exposed pregnant Sprague-Dawley rats to 500 ppb arsenic (as arsenite) via the drinking water. Subtracted cDNA libraries comparing control to arsenic exposed embryonic lungs were generated. In addition, a broad Western blot analysis was performed to identify altered protein expression. A total of 59 genes and 34 proteins were identified as being altered. Pathway mapping and analysis showed that cell motility was the process most af

SUBMITTER: Petrick JS 

PROVIDER: S-EPMC2882184 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature