Reduced expression of the v-SNAREs AtVAMP71/AtVAMP7C gene family in Arabidopsis reduces drought tolerance by suppression of abscisic acid-dependent stomatal closure.

ABSTRACT: Stomatal closure during water stress is a major plant mechanism for reducing the loss of water through leaves. The opening and closure of stomata are mediated by endomembrane trafficking. The role of the vacuolar trafficking pathway, that involves v-SNAREs of the AtVAMP71 family (formerly called AtVAMP7C) in stomatal movements, was analysed. Expression of AtVAMP711-14 genes was manipulated in Arabidopsis plants with sense or antisense constructs by transformation of the AtVAMP711 gene. Antisense plants exhibited decreased stomatal closure during drought or after treatment with abscisic acid (ABA), resulting in the rapid loss of leaf water and tissue collapse. No improvement was seen in plants overexpressing the AtVAMP711 gene, suggesting that wild-type levels of AtVAMP711 expression are su

SUBMITTER: Leshem Y 

PROVIDER: S-EPMC2882261 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature