Protein binding hot spots and the residue-residue pairing preference: a water exclusion perspective.

ABSTRACT:

Background

A protein binding hot spot is a small cluster of residues tightly packed at the center of the interface between two interacting proteins. Though a hot spot constitutes a small fraction of the interface, it is vital to the stability of protein complexes. Recently, there are a series of hypotheses proposed to characterize binding hot spots, including the pioneering O-ring theory, the insightful 'coupling' and 'hot region' principle, and our 'double water exclusion' (DWE) hypothesis. As the perspective changes from the O-ring theory to the DWE hypothesis, we examine the physicochemical properties of the binding hot spots under the new hypothesis and compare with those under the O-ring theory.

Results

The requirements for a cluster of residues to form a hot spot under

SUBMITTER: Liu Q 

PROVIDER: S-EPMC2882391 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature