Shotgun proteomics identifies proteins specific for acute renal transplant rejection.

ABSTRACT:

Purpose

Acute rejection (AR) remains the primary risk factor for renal transplant outcome; development of non-invasive diagnostic biomarkers for AR is an unmet need.

Experimental design

We used shotgun proteomics applying LC-MS/MS and ELISA to analyze a set of 92 urine samples, from patients with AR, stable grafts (STA), proteinuria (NS), and healthy controls.

Results

A total of 1446 urinary proteins (UP) were identified along with a number of nonspecific proteinuria-specific, renal transplantation specific and AR-specific proteins. Relative abundance of identified UP was measured by protein-level spectral counts adopting a weighted fold-change statistic, assigning increased weight for more frequently observed proteins. We have identified alterations in a number of sp

SUBMITTER: Sigdel TK 

PROVIDER: S-EPMC2883247 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature