Hedgehog is an anti-inflammatory epithelial signal for the intestinal lamina propria.

ABSTRACT:

Background & aims

Epithelial Hedgehog (Hh) ligands regulate several aspects of fetal intestinal organogenesis, and emerging data implicate the Hh pathway in inflammatory signaling in the adult colon. Here, we investigated the effects of chronic Hh inhibition in vivo and profiled molecular pathways acutely modulated by Hh signaling in the intestinal mesenchyme.

Methods

The progression of inflammatory disease was characterized in a bi-transgenic mouse model of chronic Hh inhibition (VFHhip). In parallel, microarray and bioinformatic analyses (Gene Ontology terms overrepresentation analysis, hierarchical clustering, and MeSH term filtration) were performed on isolated cultured intestinal mesenchyme acutely exposed to Hh ligand.

Results

Six- to 10-month-old VFHhip animals

SUBMITTER: Zacharias WJ 

PROVIDER: S-EPMC2883680 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature