Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry.

ABSTRACT:

Background

Biofuels derived from algae biomass and algae lipids might reduce dependence on fossil fuels. Existing analytical techniques need to facilitate rapid characterization of algal species by phenotyping hydrocarbon-related constituents.

Results

In this study, we compared the hydrocarbon rich algae Botryococcus braunii against the photoautotrophic model algae Chlamydomonas reinhardtii using pyrolysis-gas chromatography quadrupole mass spectrometry (pyGC-MS). Sequences of up to 48 dried samples can be analyzed using pyGC-MS in an automated manner without any sample preparation. Chromatograms of 30-min run times are sufficient to profile pyrolysis products from C8 to C40 carbon chain length. The freely available software tools AMDIS and SpectConnect enables straightforwa

SUBMITTER: Barupal DK 

PROVIDER: S-EPMC2883956 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature