G-stack modulated probe intensities on expression arrays - sequence corrections and signal calibration.

ABSTRACT:

Background

The brightness of the probe spots on expression microarrays intends to measure the abundance of specific mRNA targets. Probes with runs of at least three guanines (G) in their sequence show abnormal high intensities which reflect rather probe effects than target concentrations. This G-bias requires correction prior to downstream expression analysis.

Results

Longer runs of three or more consecutive G along the probe sequence and in particular triple degenerated G at its solution end ((GGG)1-effect) are associated with exceptionally large probe intensities on GeneChip expression arrays. This intensity bias is related to non-specific hybridization and affects both perfect match and mismatch probes. The (GGG)1-effect tends to increase gradually for microarrays of late

SUBMITTER: Fasold M 

PROVIDER: S-EPMC2884167 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature