Heterogeneous diffusion of a membrane-bound pHLIP peptide.

ABSTRACT: Lateral diffusion of cell membrane constituents is a prerequisite for many biological functions. However, the diffusivity (or mobility) of a membrane-bound species can be influenced by many factors. To provide a better understanding of how the conformation and location of a membrane-bound biological molecule affect its mobility, herein we study the diffusion properties of a pH low insertion peptide (pHLIP) in model membranes using fluorescence correlation spectroscopy. It is found that when the pHLIP peptide is located on the membrane surface, its lateral diffusion is characterized by a distribution of diffusion times, the characteristic of which depends on the peptide/lipid ratio. Whereas, under conditions where pHLIP adopts a well-defined transmembrane alpha-helical conformation the pept

SUBMITTER: Guo L 

PROVIDER: S-EPMC2884227 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature