Using a single fluorescent reporter gene to infer half-life of extrinsic noise and other parameters of gene expression.

ABSTRACT: Fluorescent and luminescent proteins are often used as reporters of transcriptional activity. Given the prevalence of noise in biochemical systems, the time-series data arising from these is of significant interest in efforts to calibrate stochastic models of gene expression and obtain information about sources of nongenetic variability. We present a statistical inference framework that can be used to estimate kinetic parameters of gene expression, as well as the strength and half-life of extrinsic noise from single fluorescent-reporter-gene time-series data. The method takes into account stochastic variability in a fluorescent signal resulting from intrinsic noise of gene expression, kinetics of fluorescent protein maturation, and extrinsic noise, which is assumed to arise at transcriptio

SUBMITTER: Komorowski M 

PROVIDER: S-EPMC2884236 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature