Millisecond timescale dynamics of human liver fatty acid binding protein: testing of its relevance to the ligand entry process.

ABSTRACT: For over a decade, scientists have been attempting to know more about the conformational dynamics of fatty acid binding proteins (FABPs), to answer the puzzling question of how ligands could access the internalized binding site(s). Conformational exchange of FABPs on the microsecond to millisecond timescales has been found in many FABPs and offers an important hypothesis for the ligand entry mechanism. Despite the potential significance, the validity of this hypothesis has not been verified yet. In this study, the slow dynamics of human liver fatty acid binding protein (hLFABP) that was shown previously to be highly flexible on millisecond timescales was quantitatively characterized in detail. In addition, the interaction between hLFABP and 1,8-ANS was studied using NMR spectroscopy, and t

SUBMITTER: Long D 

PROVIDER: S-EPMC2884256 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature