Analysis of microRNA transcriptome by deep sequencing of small RNA libraries of peripheral blood.

ABSTRACT:

Background

MicroRNAs are a class of small non-coding RNAs that regulate mRNA expression at the post - transcriptional level and thereby many fundamental biological processes. A number of methods, such as multiplex polymerase chain reaction, microarrays have been developed for profiling levels of known miRNAs. These methods lack the ability to identify novel miRNAs and accurately determine expression at a range of concentrations. Deep or massively parallel sequencing methods are providing suitable platforms for genome wide transcriptome analysis and have the ability to identify novel transcripts.

Results

The results of analysis of small RNA sequences obtained by Solexa technology of normal peripheral blood mononuclear cells, tumor cell lines K562 and HL60 are presented. In ge

SUBMITTER: Vaz C 

PROVIDER: S-EPMC2885365 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature