Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (Laodelphax striatellus), a vector insect transmitting rice stripe virus (RSV).

ABSTRACT:

Background

The small brown planthopper (Laodelphax striatellus) is an important agricultural pest that not only damages rice plants by sap-sucking, but also acts as a vector that transmits rice stripe virus (RSV), which can cause even more serious yield loss. Despite being a model organism for studying entomology, population biology, plant protection, molecular interactions among plants, viruses and insects, only a few genomic sequences are available for this species. To investigate its transcriptome and determine the differences between viruliferous and naïve L. striatellus, we employed 454-FLX high-throughput pyrosequencing to generate EST databases of this insect.

Results

We obtained 201,281 and 218,681 high-quality reads from viruliferous and naïve L. striatellus, respec

SUBMITTER: Zhang F 

PROVIDER: S-EPMC2885366 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature