Noninvasive tracking of donor cell homing by near-infrared fluorescence imaging shortly after bone marrow transplantation.

ABSTRACT:

Background

Many diseases associated with bone marrow transplantation (BMT) are caused by transplanted hematopoietic cells, and the onset of these diseases occurs after homing of donor cells in the initial phase after BMT. Noninvasive observation of donor cell homing shortly after transplantation is potentially valuable for improving therapeutic outcomes of BMT by diagnosing the early stages of these diseases.

Methodology/principal findings

Freshly harvested near-infrared fluorescence-labeled cells were noninvasively observed for 24 h after BMT using a photon counting device to track their homing process. In a congenic BMT model, the homing of Alexa Fluor 750-labeled donor cells in the tibia was detected less than 1 h after BMT. In addition, subsequent cell distribution in an

SUBMITTER: Ushiki T 

PROVIDER: S-EPMC2885427 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature