Ontology highlight
ABSTRACT:
SUBMITTER: Goldberg AD
PROVIDER: S-EPMC2885838 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Cell 20100301 5
The incorporation of histone H3 variants has been implicated in the epigenetic memory of cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we report genome-wide profiles of H3 variants in mammalian embryonic stem cells and neuronal precursor cells. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. The H3.3 chaperone Hira is required for H3.3 enrichment at active and r ...[more]