Cleavable C-terminal His-tag vectors for structure determination.

ABSTRACT: High-throughput structural genomics projects seek to delineate protein structure space by determining the structure of representatives of all major protein families. Generally this is accomplished by processing numerous proteins through standardized protocols, for the most part involving purification of N-terminally His-tagged proteins. Often proteins that fail this approach are abandoned, but in many cases further effort is warranted because of a protein's intrinsic value. In addition, failure often occurs relatively far into the path to structure determination, and many failed proteins passed the first critical step, expression as a soluble protein. Salvage pathways seek to recoup the investment in this subset of failed proteins through alternative cloning, nested truncations, chemical m

SUBMITTER: Eschenfeldt WH 

PROVIDER: S-EPMC2885959 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature