Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples.

ABSTRACT:

Background

Given the epidemic proportions of obesity worldwide and the concurrent prevalence of metabolic syndrome, there is an urgent need for better understanding the underlying mechanisms of metabolic syndrome, in particular, the gene expression differences which may participate in obesity, insulin resistance and the associated series of chronic liver conditions. Real-time PCR (qRT-PCR) is the standard method for studying changes in relative gene expression in different tissues and experimental conditions. However, variations in amount of starting material, enzymatic efficiency and presence of inhibitors can lead to quantification errors. Hence the need for accurate data normalization is vital. Among several known strategies for data normalization, the use of reference genes as

SUBMITTER: Mehta R 

PROVIDER: S-EPMC2886049 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature