Transcript and protein profiling identify candidate gene sets of potential adaptive significance in New Zealand Pachycladon.

ABSTRACT:

Background

Transcript profiling of closely related species provides a means for identifying genes potentially important in species diversification. However, the predictive value of transcript profiling for inferring downstream-physiological processes has been unclear. In the present study we use shotgun proteomics to validate inferences from microarray studies regarding physiological differences in three Pachycladon species. We compare transcript and protein profiling and evaluate their predictive value for inferring glucosinolate chemotypes characteristic of these species.

Results

Evidence from heterologous microarrays and shotgun proteomics revealed differential expression of genes involved in glucosinolate hydrolysis (myrosinase-associated proteins) and biosynthesis (meth

SUBMITTER: Voelckel C 

PROVIDER: S-EPMC2886070 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature