New assembly, reannotation and analysis of the Entamoeba histolytica genome reveal new genomic features and protein content information.

ABSTRACT:

Background

In order to maintain genome information accurately and relevantly, original genome annotations need to be updated and evaluated regularly. Manual reannotation of genomes is important as it can significantly reduce the propagation of errors and consequently diminishes the time spent on mistaken research. For this reason, after five years from the initial submission of the Entamoeba histolytica draft genome publication, we have re-examined the original 23 Mb assembly and the annotation of the predicted genes.

Principal findings

The evaluation of the genomic sequence led to the identification of more than one hundred artifactual tandem duplications that were eliminated by re-assembling the genome. The reannotation was done using a combination of manual and automated

SUBMITTER: Lorenzi HA 

PROVIDER: S-EPMC2886108 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature