Filaggrin-deficient mice exhibit TH17-dominated skin inflammation and permissiveness to epicutaneous sensitization with protein antigen.

ABSTRACT:

Background

Filaggrin is important for skin barrier function and is mutated in 15% to 20% of patients with atopic dermatitis.

Objective

To examine whether filaggrin deficiency predisposes to skin inflammation and epicutaneous sensitization with protein antigen.

Methods

Skin histology in filaggrin-deficient flaky tail (ft)/ft mice and wild-type controls was assessed by Hematoxylin and Eosin (H&E) staining and immunohistochemistry. Cytokine mRNA expression was examined by quantitative RT-PCR. Serum antibody levels and splenocyte secretion of cytokines were measured by ELISA.

Results

The ft/ft mice developed eczematous skin lesions after age 28 weeks and a progressive increase in serum IgE and IgG(1) levels. Normal-appearing skin from 8-week-old ft/ft mice had epid

SUBMITTER: Oyoshi MK 

PROVIDER: S-EPMC2886150 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature