The spatial expression and regulation of transcription factors IDEF1 and IDEF2.

ABSTRACT:

Background and aims

Under conditions of low iron availability, rice plants induce genes involved in iron uptake and utilization. The iron deficiency-responsive cis-acting element binding factors 1 and 2 (IDEF1 and IDEF2) regulate transcriptional response to iron deficiency in rice roots. Clarification of the functions of IDEF1 and IDEF2 could uncover the gene regulation mechanism.

Methods

Spatial patterns of IDEF1 and IDEF2 expression were analysed by histochemical staining of IDEF1 and IDEF2 promoter-GUS transgenic rice lines. Expression patterns of the target genes of IDEF1 and IDEF2 were analysed using transformants with induced or repressed expression of IDEF1 or IDEF2 grown in iron-rich or in iron-deficient solutions for 1 d.

Key results

IDEF1 and IDEF2 were high

SUBMITTER: Kobayashi T 

PROVIDER: S-EPMC2887061 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature