Molecular cloning and gene expression analysis of the leptin receptor in the Chinese mitten crab Eriocheir sinensis.

ABSTRACT:

Background

Leptin is an adipocyte-derived hormone with multiple functions that regulates energy homeostasis and reproductive functions. Increased knowledge of leptin receptor function will enhance our understanding of the physiological roles of leptin in animals.

Methodology/principal findings

In the present study, a full-length leptin receptor (lepr) cDNA, consisting of 1,353 nucleotides, was cloned from Chinese mitten crab (Eriocheir sinensis) using rapid amplification of cDNA ends (RACE) following the identification of a single expressed sequence tag (EST) clone in a cDNA library. The lepr cDNA consisted of a 22-nucleotide 5'-untranslated region (5' UTR), a 402-nucleotide open reading frame (ORF) and a 929-nucleotide 3' UTR. Multiple sequence alignments revealed that Chin

SUBMITTER: Jiang H 

PROVIDER: S-EPMC2887359 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature