Population-level transcriptome sequencing of nonmodel organisms Erynnis propertius and Papilio zelicaon.

ABSTRACT:

Background

Several recent studies have demonstrated the use of Roche 454 sequencing technology for de novo transcriptome analysis. Low error rates and high coverage also allow for effective SNP discovery and genetic diversity estimates. However, genetically diverse datasets, such as those sourced from natural populations, pose challenges for assembly programs and subsequent analysis. Further, estimating the effectiveness of transcript discovery using Roche 454 transcriptome data is still a difficult task.

Results

Using the Roche 454 FLX Titanium platform, we sequenced and assembled larval transcriptomes for two butterfly species: the Propertius duskywing, Erynnis propertius (Lepidoptera: Hesperiidae) and the Anise swallowtail, Papilio zelicaon (Lepidoptera: Papilionidae). Th

SUBMITTER: O'Neil ST 

PROVIDER: S-EPMC2887415 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature