Paucity and preferential suppression of transgenes in late replication domains of the D. melanogaster genome.

ABSTRACT:

Background

Eukaryotic genomes are organized in extended domains with distinct features intimately linking genome structure, replication pattern and chromatin state. Recently we identified a set of long late replicating euchromatic regions that are underreplicated in salivary gland polytene chromosomes of D. melanogaster.

Results

Here we demonstrate that these underreplicated regions (URs) have a low density of P-element and piggyBac insertions compared to the genome average or neighboring regions. In contrast, Minos-based transposons show no paucity in URs but have a strong bias to testis-specific genes. We estimated the suppression level in 2,852 stocks carrying a single P-element by analysis of eye color determined by the mini-white marker gene and demonstrate that the pro

SUBMITTER: Babenko VN 

PROVIDER: S-EPMC2887417 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature