Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus.

ABSTRACT:

Background

Current vaccines to avian influenzae virus (AIV), a highly contagious disease of birds, need to be constantly updated due to the high level of variation in the target antigens. Therefore, a vaccine that could induce broad cross protection against AIV is required. The M2 membrane protein is structurally conserved amongst AIV subtypes but tends in induce a poor immune response, whereas C3d has been shown in many species to enhance immunogenicity. In this study, we investigated the potential of M2-avian C3d fusion proteins to provide effective immunity.

Results

We fused chicken complement C3d to sM2 (M2 protein with the transmembrane region deleted) of AIV and expressed four fusion proteins, GST (Glutathione S-transferase tagged proteins in pGEX expression vector) -C

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC2887435 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature