A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation.

ABSTRACT:

Background

DNA methylation changes are widely used as early molecular markers in cancer detection. Sensitive detection and classification of rare methylation changes in DNA extracted from circulating body fluids or complex tissue samples is crucial for the understanding of tumor etiology, clinical diagnosis and treatment. In this paper, we describe a combined method to monitor the presence of methylated tumor DNA in an excess of unmethylated background DNA of non-tumorous cells. The method combines heavy methyl-PCR, which favors preferential amplification of methylated marker sequence from bisulfite-treated DNA with a methylation-specific single nucleotide primer extension monitored by ion-pair, reversed-phase, high-performance liquid chromatography separation.

Results

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SUBMITTER: Tierling S 

PROVIDER: S-EPMC2887863 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature